Z. Hong Zhou, Ph.D.



6350A CNSI

Director, Electron Imaging Center for NanoMachines (EICN)
Professor, Microbiology, Immunology & Molecular Genetics)
Member, Biochemistry, Biophysics & Structural Biology GPB Home Area, California NanoSystems Institute, Immunity, Microbes & Molecular Pathogenesis GPB Home Area
Research Interests
Structure and function of molecular complexes, viruses and cellular machineries
Professor Hong Zhou’s lab focuses on 3D structural studies of biological complexes using cryo-electron microscopy (cryoEM) and cryo-electron tomography (cryoET). These emerging methods are particularly suitable for structure determination of large molecular complexes, viruses, cellular machineries and bacterial cells. Recent efforts have focuses on developing and applying advanced cryoEM and cryoET techniques to visualize the dynamic processes of microbial infections and to decipher the mechanisms of fundamental biological processes. Dr. Zhou’s group is at the forefront in pushing the envelope of cryoEM reconstruction to atomic resolution.

Technical Research Interests
Recent advances have given cryo-electron microscopy and single-particle reconstruction (“cryoEM”) an increasingly important role in determining, at 4-7 Å resolution, the 3D structures of macromolecular complexes or biological nanomachines (~150 kDa and 10 nm in dimension). At this resolution, secondary structural elements and bulky amino-acid side chains are readily resolved and can be used to build pseudoatomic models of its subunits for understanding molecular interactions. The emerging method of cryo-electron tomography (cryoET) allows the determination of three dimensional architectures of objects ranging in size from a nanometer to micrometers. These structural methods provide exciting opportunities to determine the structures of microbes and subcellular assemblies that are either too large or too heterogeneous to be investigated by conventional crystallographic or NMR methods. Research in my laboratory aims to understand the mechanisms governing macromolecular functions by pushing the resolution limit of cryoEM to near-atomic resolution and by describing large, pleomorphic, dynamic structures or conformations using the integrative approach of cryoEM and cryoET. The long-term goal of our research is to study their structures and mechanisms of actions by an integrative approach using cryoEM/cryoET, bioinformatics modeling and high performance computing. Current efforts focus on three intermediate projects toward our goal: 1, to develop integrative techniques for atomic resolution cryoEM structural determination; 2, to decipher the mechanisms of actions of multi-enzyme complexes and multi-component nanomachines, such as human pyruvate dehydrogenase complexes; 3, to establish a 3D atlas of key events leading to the assembly and host infection of tumor herpesviruses.

Z. Hong Zhou, Ph. D. Professor Hong Zhou is a Professor in the Microbiology, Immunology, and Molecular Genetics department. His group studies the structures and functions of membrane proteins, protein-nucleic acid complexes and microbes using cryo electron microscopy (cryoEM) and cryo electron tomography (cryoET). He combines expertise in structural biology, microbiology, computational biology, and bioinformatics to address fundamental questions in biology. Recent efforts have focused on membrane protein structures and mechanisms of gene replication and transcription. Dr. Zhou was a Pew Scholar in the Biomedical Sciences, received numerous awards, including the Basil O’Connor Scholar Award, the Established Investigator Award of American Heart Association, the Burton award, and the KH Kuo Award of Distinguished Scientist.

Zhang Xing, Patel Avnish, Celma Cristina C, Yu Xuekui, Roy Polly, Zhou Z Hong, Atomic model of a nonenveloped virus reveals pH sensors for a coordinated process of cell entry, Nature structural & molecular biology, 2016, 23 (1), 74-80.

Toso Daniel B, Javed Muhammad Mohsin, Czornyj Elizabeth, Gunsalus Robert P, Zhou Z Hong, Discovery and Characterization of Iron Sulfide and Polyphosphate Bodies Coexisting in Archaeoglobus fulgidus Cells, Archaea (Vancouver, B.C.), 2016, 2016 (26), 4706532.
Lee Grace, Chakraborty Urmi, Gebhart Dana, Govoni Gregory R, Zhou Z Hong, Scholl Dean, F-type bacteriocins of Listeria: a new class of phage tail-like structures reveals broad parallel co-evolution between tailed bacteriophages and high molecular weight bacteriocins, Journal of bacteriology, 2016.
Bor Batbileg, Poweleit Nicole, Bois Justin S, Cen Lujia, Bedree Joseph K, Zhou Z Hong, Gunsalus Robert P, Lux Renate, McLean Jeffrey S, He Xuesong, Shi Wenyuan, Phenotypic and Physiological Characterization of the Epibiotic Interaction Between TM7x and Its Basibiont Actinomyces, Microbial ecology, 2016, 71 (1), 243-55.
Mayle Kristine M, Dern Kathryn R, Wong Vincent K, Sung Shijun, Ding Ke, Rodriguez April R, Taylor Zachary, Zhou Z Hong, Grundfest Warren S, Deming Timothy J, Kamei Daniel T, Polypeptide-Based Gold Nanoshells for Photothermal Therapy,Journal of laboratory automation, 2016, 120 (26).
Huber Rachel C, Ferreira Amy S, Aguirre Jordan C, Kilbride Daniel, Toso Daniel B, Mayoral Kenny, Zhou Z Hong, Kopidakis Nikos, Rubin Yves, Schwartz Benjamin J, Mason Thomas G, Tolbert Sarah H, Structure and Conductivity of Semiconducting Polymer Hydrogels, The journal of physical chemistry. B, 2016, 120 (26), 6215-24.
Huynh Kevin W, Cohen Matthew R, Jiang Jiansen, Samanta Amrita, Lodowski David T, Zhou Z Hong, Moiseenkova-Bell Vera Y, Structure of the full-length TRPV2 channel by cryo-EM, Nature communications, 2016, 7 (26), 11130.
Dejnirattisai Wanwisa, Wongwiwat Wiyada, Supasa Sunpetchuda, Zhang Xiaokang, Dai Xinghong, Rouvinski Alexander, Rouvinsky Alexander, Jumnainsong Amonrat, Edwards Carolyn, Quyen Nguyen Than Ha, Duangchinda Thaneeya, Grimes Jonathan M, Tsai Wen-Yang, Lai Chih-Yun, Wang Wei-Kung, Malasit Prida, Farrar Jeremy, Simmons Cameron P, Zhou Z Hong, Rey Felix A, Mongkolsapaya Juthathip, Screaton Gavin R, A new class of highly potent, broadly neutralizing antibodies isolated from viremic patients infected with dengue virus, Nature immunology, 2015, 16 (2), 170-7.
Yu Xuekui, Jiang Jiansen, Sun Jingchen, Zhou Z Hong, A putative ATPase mediates RNA transcription and capping in a dsRNA virus, eLife, 2015, 4.
Clemens Daniel L, Ge Peng, Lee Bai-Yu, Horwitz Marcus A, Zhou Z Hong, Atomic structure of T6SS reveals interlaced array essential to function, Cell, 2015, 160 (5), 940-51.
Jiang Jiansen, Pentelute Bradley L, Collier R John, Zhou Z Hong, Atomic structure of anthrax protective antigen pore elucidates toxin translocation, Nature, 2015, 521 (7553), 545-9.
Ge Peng, Scholl Dean, Leiman Petr G, Yu Xuekui, Miller Jeff F, Zhou Z Hong, Atomic structures of a bactericidal contractile nanotube in its pre- and postcontraction states, Nature structural & molecular biology, 2015, 22 (5), 377-82.
AhYoung Andrew P, Jiang Jiansen, Zhang Jiang, Khoi Dang Xuan, Loo Joseph A, Zhou Z Hong, Egea Pascal F, Conserved SMP domains of the ERMES complex bind phospholipids and mediate tether assembly, Proceedings of the National Academy of Sciences of the United States of America, 2015, 112 (25), E3179-88.
Dai Xinghong, Gong Danyang, Xiao Yuchen, Wu Ting-Ting, Sun Ren, Zhou Z Hong, CryoEM and mutagenesis reveal that the smallest capsid protein cements and stabilizes Kaposi’s sarcoma-associated herpesvirus capsid, Proceedings of the National Academy of Sciences of the United States of America, 2015, 112 (7), E649-56.
Kaweeteerawat Chitrada, Chang Chong Hyun, Roy Kevin R, Liu Rong, Li Ruibin, Toso Daniel, Fischer Heidi, Ivask Angela, Ji Zhaoxia, Zink Jeffrey I, Zhou Z Hong, Chanfreau Guillaume Francois, Telesca Donatello, Cohen Yoram, Holden Patricia Ann, Nel Andre E, Godwin Hilary A, Cu Nanoparticles Have Different Impacts in Escherichia coli and Lactobacillus brevis than Their Microsized and Ionic Analogues, ACS nano, 2015, 9 (7), 7215-25.
Zhang Xing, Ding Ke, Yu Xuekui, Chang Winston, Sun Jingchen, Zhou Z Hong, In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus, Nature, 2015, 527 (7579), 531-4.
Huber Rachel C, Ferreira Amy S, Thompson Robert, Kilbride Daniel, Knutson Nicholas S, Devi Lekshmi Sudha, Toso Daniel B, Challa J Reddy, Zhou Z Hong, Rubin Yves, Schwartz Benjamin J, Tolbert Sarah H, POLARON DYNAMICS. Long-lived photoinduced polaron formation in conjugated polyelectrolyte-fullerene assemblies, Science (New York, N.Y.), 2015, 348 (6241), 1340-3.
Jurado Ashley R, Huang Christine S, Zhang Xing, Zhou Z Hong, Tong Liang, Structure and substrate selectivity of the 750-kDa α6β6 holoenzyme of geranyl-CoA carboxylase, Nature communications, 2015, 6 (7579), 8986.
Jiang Jiansen, Chan Henry, Cash Darian D, Miracco Edward J, Ogorzalek Loo Rachel R, Upton Heather E, Cascio Duilio, O’Brien Johnson Reid, Collins Kathleen, Loo Joseph A, Zhou Z Hong, Feigon Juli, Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions, Science (New York, N.Y.), 2015, 350 (6260), aab4070.
Volland Stefanie, Hughes Louise C, Kong Christina, Burgess Barry L, Linberg Kenneth A, Luna Gabriel, Zhou Z Hong, Fisher Steven K, Williams David S, Three-dimensional organization of nascent rod outer segment disk membranes,Proceedings of the National Academy of Sciences of the United States of America, 2015, 112 (48), 14870-5.
Sathiyamoorthy Karthik, Jiang Jiansen, Hu Yao Xiong, Rowe Cynthia L, Möhl Britta S, Chen Jia, Jiang Wei, Mellins Elizabeth D, Longnecker Richard, Zhou Z Hong, Jardetzky Theodore S, Assembly and architecture of the EBV B cell entry triggering complex, PLoS pathogens, 2014, 10 (8), e1004309.
Yang Kui, Wills Elizabeth, Lim Han Young, Zhou Z Hong, Baines Joel D, Association of herpes simplex virus pUL31 with capsid vertices and components of the capsid vertex-specific complex, Journal of virology, 2014, 88 (7), 3815-25.
Buehler Daniel C, Marsden Matthew D, Shen Sean, Toso Daniel B, Wu Xiaomeng, Loo Joseph A, Zhou Z Hong, Kickhoefer Valerie A, Wender Paul A, Zack Jerome A, Rome Leonard H, Bioengineered vaults: self-assembling protein shell-lipophilic core nanoparticles for drug delivery, ACS nano, 2014, 8 (8), 7723-32.
Ge Peng, Zhou Z Hong, Chaperone fusion proteins aid entropy-driven maturation of class II viral fusion proteins, Trends in microbiology, 2014, 22 (2), 100-6.
Ge Peng, Durer Zeynep A Oztug, Kudryashov Dmitri, Zhou Z Hong, Reisler Emil, Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments, Nature structural & molecular biology, 2014, 21 (12), 1075-81.
Zhou Z Hong, Hui Wong Hoi, Shah Sanket, Jih Jonathan, O’Connor Christine M, Sherman Michael B, Kedes Dean H, Schein Stan, Four levels of hierarchical organization, including noncovalent chainmail, brace the mature tumor herpesvirus capsid against pressurization, Structure (London, England : 1993), 2014, 22 (10), 1385-98.
Gurel Pinar S, Ge Peng, Grintsevich Elena E, Shu Rui, Blanchoin Laurent, Zhou Z Hong, Reisler Emil, Higgs Henry N, INF2-mediated severing through actin filament encirclement and disruption, Current biology : CB, 2014, 24 (2), 156-64.
Ren Shuxun, Lu Gang, Ota Asuka, Zhou Z Hong, Vondriska Thomas M, Lane Timothy F, Wang Yibin, IRE1 phosphatase PP2Ce regulates adaptive ER stress response in the postpartum mammary gland, PloS one, 2014, 9 (11), e111606.
Dai Xinghong, Gong Danyang, Wu Ting-Ting, Sun Ren, Zhou Z Hong, Organization of capsid-associated tegument components in Kaposi’s sarcoma-associated herpesvirus, Journal of virology, 2014, 88 (21), 12694-702.
Mrazek Jan, Toso Daniel, Ryazantsev Sergey, Zhang Xing, Zhou Z Hong, Fernandez Beatriz Campo, Kickhoefer Valerie A, Rome Leonard H, Polyribosomes are molecular 3D nanoprinters that orchestrate the assembly of vault particles, ACS nano, 2014, 8 (11), 11552-9.
Yan Xiaodong, Cardone Giovanni, Zhang Xing, Zhou Z Hong, Baker Timothy S, Single particle analysis integrated with microscopy: a high-throughput approach for reconstructing icosahedral particles, Journal of structural biology, 2014, 186 (1), 8-18.
Zhou Z Hong, Structures of viral membrane proteins by high-resolution cryoEM, Current opinion in virology, 2014, 5 (8), 111-9.
Yu Xuekui, Jin Lei, Jih Jonathan, Shih Chiaho, Zhou Z Hong, 3.5Å cryoEM structure of hepatitis B virus core assembled from full-length core protein, PloS one, 2013, 8 (9), e69729.
Zhang Xing, Guo Huatao, Jin Lei, Czornyj Elizabeth, Hodes Asher, Hui Wong H, Nieh Angela W, Miller Jeff F, Zhou Z Hong, A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 A resolution, eLife, 2013, 2 (11), e01299.
Zhang Xiaokang, Ge Peng, Yu Xuekui, Brannan Jennifer M, Bi Guoqiang, Zhang Qinfen, Schein Stan, Zhou Z Hong, Cryo-EM structure of the mature dengue virus at 3.5-Å resolution, Nature structural & molecular biology, 2013, 20 (1), 105-10.
Jiang Ziyue Karen, Koh Sok Boon S, Sato Makoto, Atanasov Ivo C, Johnson Mai, Zhou Z Hong, Deming Timothy J, Wu Lily, Engineering polypeptide coatings to augment gene transduction and in vivo stability of adenoviruses, Journal of controlled release : official journal of the Controlled Release Society, 2013, 166 (1), 75-85.
Slaymaker Ian M, Fu Yang, Toso Daniel B, Ranatunga Nimna, Brewster Aaron, Forsburg Susan L, Zhou Z Hong, Chen Xiaojiang S, Mini-chromosome maintenance complexes form a filament to remodel DNA structure and topology, Nucleic acids research, 2013, 41 (5), 3446-56.
Lu Gang, Ota Asuka, Ren Shuxun, Franklin Sarah, Rau Christoph D, Ping Peipei, Lane Timothy F, Zhou Z Hong, Reue Karen, Lusis Aldons J, Vondriska Thomas, Wang Yibin, PPM1l encodes an inositol requiring-protein 1 (IRE1) specific phosphatase that regulates the functional outcome of the ER stress response, Molecular metabolism, 2013, 2 (4), 405-16.
Hui Wong H, Tang Qiyi, Liu Hongrong, Atanasov Ivo, Liu Fenyong, Zhu Hua, Zhou Z Hong, Protein interactions in the murine cytomegalovirus capsid revealed by cryoEM, Protein & cell, 2013, 4 (11), 833-45.
Jiang Jiansen, Magilnick Nathaniel, Tsirulnikov Kirill, Abuladze Natalia, Atanasov Ivo, Ge Peng, Narla Mohandas, Pushkin Alexander, Zhou Z Hong, Kurtz Ira, Single particle electron microscopy analysis of the bovine anion exchanger 1 reveals a flexible linker connecting the cytoplasmic and membrane domains, PloS one, 2013, 8 (2), e55408.
Hong Kyungah, Upton Heather, Miracco Edward J, Jiang Jiansen, Zhou Z Hong, Feigon Juli, Collins Kathleen, Tetrahymena telomerase holoenzyme assembly, activation, and inhibition by domains of the p50 central hub, Molecular and cellular biology, 2013, 33 (19), 3962-71.
Jiang Jiansen, Miracco Edward J, Hong Kyungah, Eckert Barbara, Chan Henry, Cash Darian D, Min Bosun, Zhou Z Hong, Collins Kathleen, Feigon Juli, The architecture of Tetrahymena telomerase holoenzyme, Nature, 2013, 496 (7444), 187-92.
Dai Xinghong, Yu Xuekui, Gong Hao, Jiang Xiaohong, Abenes Gerrado, Liu Hongrong, Shivakoti Sakar, Britt William J, Zhu Hua, Liu Fenyong, Zhou Z Hong, The smallest capsid protein mediates binding of the essential tegument protein pp150 to stabilize DNA-containing capsids in human cytomegalovirus, PLoS pathogens, 2013, 9 (8), e1003525.
Huang Christine S, Ge Peng, Zhou Z Hong, Tong Liang, An unanticipated architecture of the 750-kDa α6β6 holoenzyme of 3-methylcrotonyl-CoA carboxylase, Nature, 2012, 481 (7380), 219-23.
Zhang Xing, Guo Huatao, Jin Lei, Xu Ming, Miller Jeffery F, Zhou Z Hong, Atomic Structure of Bordetella Bacteriophage Reveals a Jellyroll Fold in CementProtein and a Topologically Distinct HK97-like Fold in Major Capsid Protein, Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 2012, 18 (Suppl 2), 72-73.
Luo Jinquan, Obmolova Galina, Malia Thomas J, Wu Sheng-Jiun, Duffy Karen E, Marion James D, Bell Jessica K, Ge Peng, Zhou Z Hong, Teplyakov Alexey, Zhao Yonghong, Lamb Roberta J, Jordan Jarrat L, San Mateo Lani R, Sweet Raymond W, Gilliland Gary L, Lateral clustering of TLR3:dsRNA signaling units revealed by TLR3ecd:3Fabs quaternary structure, Journal of molecular biology, 2012, 421 (1), 112-24.
Xiao Qiangfeng, Sohn Hiesang, Chen Zheng, Toso Daniel, Mechlenburg Matthew, Zhou Z Hong, Poirier Eric, Dailly Anne, Wang Haiqiang, Wu Zhongbiao, Cai Mei, Lu Yunfeng, Mesoporous metal and metal alloy particles synthesized by aerosol-assisted confined growth of nanocrystals, Angewandte Chemie (International ed. in English), 2012, 51 (42), 10546-50.
Zhou Z Hong, Seeing engineered loops in a gene delivery vehicle by cryoEM, Structure (London, England : 1993), 2012, 20 (8), 1286-8.
Hughes Louise C, Ralston Katherine S, Hill Kent L, Zhou Z Hong, Three-dimensional structure of the Trypanosome flagellum suggests that the paraflagellar rod functions as a biomechanical spring, PloS one, 2012, 7 (1), e25700.
Tao Changlu, Xia Chenglong, Chen Xiaobing, Zhou Z Hong, Bi Guoqiang, Ultrastructural analysis of neuronal synapses using state-of-the-art nano-imaging techniques, Neuroscience bulletin, 2012, 28 (4), 321-32.
Gopal Ajaykumar, Zhou Z Hong, Knobler Charles M, Gelbart William M, Visualizing large RNA molecules in solution, RNA (New York, N.Y.), 2012, 18 (2), 284-99.
Green Todd J, Rowse Michael, Tsao Jun, Kang Jungsoon, Ge Peng, Zhou Z Hong, Luo Ming, Access to RNA encapsidated in the nucleocapsid of vesicular stomatitis virus, Journal of virology, 2011, 85 (6), 2714-22.
Yang Zhe, Hu Wei, Chen Kevin, Wang Jing, Lux Renate, Zhou Z Hong, Shi Wenyuan, Alanine 32 in PilA is important for PilA stability and type IV pili function in Myxococcus xanthus, Microbiology (Reading, England), 2011, 157 (Pt 7), 1920-8.
Meng Huan, Yang Sui, Li Zongxi, Xia Tian, Chen Justin, Ji Zhaoxia, Zhang Haiyuan, Wang Xiang, Lin Sijie, Huang Connie, Zhou Z Hong, Zink Jeffrey I, Nel Andre E, Aspect ratio determines the quantity of mesoporous silica nanoparticle uptake by a small GTPase-dependent macropinocytosis mechanism, ACS nano, 2011, 5 (6), 4434-47.
Yu Xuekui, Ge Peng, Jiang Jiansen, Atanasov Ivo, Zhou Z Hong, Atomic model of CPV reveals the mechanism used by this single-shelled virus to economically carry out functions conserved in multishelled reoviruses, Structure (London, England : 1993), 2011, 19 (5), 652-61.
Zhou Z Hong, Atomic resolution cryo electron microscopy of macromolecular complexes, Advances in protein chemistry and structural biology, 2011, 82 (6), 1-35.
Yu Xuekui, Shah Sanket, Lee Manfred, Dai Wei, Lo Pierrette, Britt William, Zhu Hua, Liu Fenyong, Zhou Z Hong, Biochemical and structural characterization of the capsid-bound tegument proteins of human cytomegalovirus, Journal of structural biology, 2011, 174 (3), 451-60.
Chen Justin, Sun Jingchen, Atanasov Ivo, Ryazantsev Sergey, Zhou Z Hong, Electron tomography reveals polyhedrin binding and existence of both empty and full cytoplasmic polyhedrosis virus particles inside infectious polyhedra, Journal of virology, 2011, 85 (12), 6077-81.
Ge Peng, Zhou Z Hong, Hydrogen-bonding networks and RNA bases revealed by cryo electron microscopy suggest a triggering mechanism for calcium switches, Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (23), 9637-42.
Zhang Xing, Zhou Z Hong, Limiting factors in atomic resolution cryo electron microscopy: no simple tricks, Journal of structural biology, 2011, 175 (3), 253-63.
Toso Daniel B, Henstra Anne M, Gunsalus Robert P, Zhou Z Hong, Structural, mass and elemental analyses of storage granules in methanogenic archaeal cells, Environmental microbiology, 2011, 13 (9), 2587-99.
Daniel C. Buehler, Daniel B. Toso, Valerie A. Kickhoefer, Z. Hong Zhou, Leonard H. Rome, Vaults Engineered for Hydrophobic Drug Delivery, Small, 2011, Epub ahead of print.
Zhang Xing, Jin Lei, Fang Qin, Hui Wong H, Zhou Z Hong, 3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry, Cell, 2010, 141 (3), 472-82.
Ming Yan, Juanjuan Du, Zhen Gu, Min Liang, Yufang Hu, Wenjun Zhang, Saul Priceman, Lily Wu, Z. Hong Zhou, Zheng Liu, Tatiana Segura, Yi Tang, Yunfeng Lu, A novel intracellular protein delivery platform based on single-protein nanocapsules, Nature Nanotechnology, 2010, 5, 48 – 53 .
Hongrong Liu, Lei Jin, Sok Boon S. Koh, Ivo Atanasov, Stan Schein, Lily Wu, Z. Hong Zhou, Atomic Structure of Human Adenovirus by Cryo-EM Reveals Interactions Among Protein Networks, Science, 2010, 329 (5995), 1038-1043.
Zhang X, Boyce M, Bhattacharya B, Zhang X, Schein S, Roy P, Zhou ZH, Bluetongue virus coat protein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins, PNAS, 2010, 107 (14), 6292-7.
Zhu Jiang, Cheng Lingpeng, Fang Qin, Zhou Z Hong, Honig Barry, Building and Refining Protein Models within Cryoelectron Microscopy Density Maps Based on Homology Modeling and Multiscale Structure Refinement, Journal of Molecular Biology, 2010, 327 (5966).
Liu J, Howell JK, Bradley SD, Zheng Y, Zhou ZH, Norris SJ, Cellular architecture of Treponema pallidum: novel flagellum, periplasmic cone, and cell envelope as revealed by cryo electron tomography, J Mol Biol, 2010, 403 (4), 546-61.
Leong PA, Yu X, Zhou ZH, Jensen GJ, Correcting for the ewald sphere in high-resolution single-particle reconstructions,Methods Enzymol, 2010, 482, 369-80.
Ge, P., Tsao, J., Schein, S., Green, T.J., Luo, M., Zhou, Z.H. , Cryo-EM Model of the Bullet-Shaped Vesicular Stomatitis Virus, Science, 2010, 327 (5966), 689-93.
Huang CS, Sadre-Bazzaz K, Shen Y, Deng B, Zhou ZH, Tong L, Crystal structure of the alpha(6)beta(6) holoenzyme of propionyl-coenzyme A carboxylase, Nature, 2010, 466 (7309), 1001-5.
Faller M, Toso D, Matsunaga M, Atanasov I, Senturia R, Chen Y, Zhou ZH, Guo F, DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures, RNA, 2010, 16 (8), 1570-83.
Jin L, Pan C, Qi Z, Zhou ZH, Jiang S, Fab crystallization and preliminary X-ray analysis of NC-1, an anti-HIV-1 antibody that recognizes the six-helix bundle core of gp41, Acta Crystallogr Sect F Struct Biol Cryst Commun, 2010, 66 (7), 854-7.
Zhang X, Zhang X, Hong Zhou Z, Low cost, high performance GPU computing solution for atomic resolution cryoEM single-particle reconstruction, J Struct Biol, 2010, 172 (3), 400-6.
Liu H, Wu L, Zhou ZH, Model of the Trimeric Fiber and Its Interactions with the Pentameric Penton Base of Human Adenovirus by Cryo-electron Microscopy, J Mol Biol, 2010, 406 (5), 764-74.
Liu S, He J, Shih C, Li K, Dai A, Zhou ZH, Zhang J, Structural comparisons of hepatitis B core antigen particles with different C-terminal lengths, Virus Res, 2010, 149 (2), 241-4.
Dai W, Hodes A, Hui WH, Gingery M, Miller JF, Zhou ZH, Three-dimensional structure of tropism-switching Bordetella bacteriophage, PNAS, 2010, 107 (9), 4347-52.
Peng, L Ryazantsev, S Sun, R Zhou, Z. H., Three-dimensional visualization of gammaherpesvirus life cycle in host cells by electron tomography, Structure, 2010, 18 (1), 47-58.
Gangalum RK, Atanasov IC, Zhou ZH, Bhat SP, {alpha}B-crystallin is found in detergent resistant membrane microdomains and is secreted via exosomes from human retinal pigment epithelial cells, J Biol Chem, 2010, 286 (5), 3261-9.
Cheng Lingpeng, Zhu Jiang, Hui Wong Hoi, Zhang Xiaokang, Honig Barry, Fang Qin, Zhou Z Hong, Backbone Model of an Aquareovirus Virion by Cryo-Electron Microscopy and Bioinformatics, Journal of molecular biology, 2009, 5 (1).
Nayak Debi P, Balogun Rilwan A, Yamada Hiroshi, Zhou Z Hong, Barman Subrata, Influenza virus morphogenesis and budding, Virus research, 2009, 143 (2), 147-61.
Guo Haitao, Wang Lili, Peng Li, Zhou Z Hong, Deng Hongyu, Open reading frame 33 of a gammaherpesvirus encodes a tegument protein essential for virion morphogenesis and egress, Journal of virology, 2009, 83 (20), 10582-95.
Li Feng, Ge Peng, Hui Wong H, Atanasov Ivo, Rogers Kestrel, Guo Qiang, Osato Daren, Falick Arnold M, Zhou Z Hong, Simpson Larry, Structure of the core editing complex (L-complex) involved in uridine insertion/deletion RNA editing in trypanosomatid mitochondria, Proceedings of the National Academy of Sciences of the United States of America, 2009, 106 (30), 12306-10.
Pan, D. X. Wang Zhou, Z. H. W. Chen C. Xu Lu, Y, Synthesis of quaternary semiconductor nanocrystals with tunable band gaps, Chemistry of Materials, 2009, 21 (12), 2489-2493.
Yu Xuekui, Jin Lei, Zhou Z Hong, 3.88 A structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy, Nature, 2008, 453 (7193), 415-9.
Jiang, J., Zhang, X., Chen, Y., Wu, Y., Zhou, Z.H., Chang, Z. and Sui, S.F., Activation of DegP chaperone-protease via formation of large cage-like oligomers upon binding to substrate proteins, PNAS, 2008, 33 (105), 11939-44.
Deng, B., O’Connor, C.M., Kedes, D.H. and Zhou, Z.H., Cryo-electron tomography of Kaposi’s sarcoma-associated herpesvirus capsids reveals dynamic scaffolding structures essential to capsid assembly and maturation, Journal of Structural Biology, 2008, 161 (3), 419-427.
Song Changyong, Jiang Huaidong, Mancuso Adrian, Amirbekian Bagrat, Peng Li, Sun Ren, Shah Sanket S, Zhou Z Hong, Ishikawa Tetsuya, Miao Jianwei, Quantitative imaging of single, unstained viruses with coherent x rays, Physical review letters, 2008, 101 (15), 158101.
Yu, X., Hiromasa, Y., Tsen, H., Stoops, J.K., Roche, T.E., Zhou, Z.H., Structures of the Human Pyruvate Dehydrogenase Complex Cores: a Highly Conserved Catalytic Center with Flexible N-terminal Domains, Structure, 2008, 16 (1), 104-114.
Cheng, L. Fang, Q. Shah, S. Atanasov, I. C. Zhou, Z. H., Subnanometer-resolution structures of the grass carp reovirus core and virion, J Mol Biol, 2008, 382 (1), 213-22.
Liu, H., Cheng,L., Zeng, S., Cai, C., Zhou,Z.H., Yang, Q., Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions, Journal of Structural Biology, 2008, 161 (1), 64-73.
Zhou, Z. H., Towards atomic resolution structural determination by single-particle cryo-electron microscopy, Curr Opin Struct Biol, 2008, 18 (2), 218-28.
Dai Wei, Jia Qingmei, Bortz Eric, Shah Sanket, Liu Jun, Atanasov Ivo, Li Xudong, Taylor Kenneth A, Sun Ren, Zhou Z Hong, Unique structures in a tumor herpesvirus revealed by cryo-electron tomography and microscopy, Journal of structural biology, 2008, 161 (3), 428-38.
Yu, X Qiao, M Atanasov, I Hu, Z Kato, T Liang, TJ Zhou, ZH, Cryo-electron microscopy and three-dimensional reconstructions of hepatitis C virus particles, Virology, 2007, 367 (1), 126-134.
Zhou, Z. H., Cypovirus, In Structure and Molecular Biology of Segmented Double-Stranded RNA Viruses, P.J.T. and M. L. Nibert eds, Horizon Scientific Press, 2007.
Deng Binbin, O’Connor Christine M, Kedes Dean H, Zhou Z Hong, Direct visualization of the putative portal in the Kaposi’s sarcoma-associated herpesvirus capsid by cryoelectron tomography, Journal of virology, 2007, 81 (7), 3640-4.
Deng, B O’Connor, CM Kedes, DH Zhou, ZH, Direct visualization of the putative portal in the Kaposi’s sarcoma-associated herpesvirus capsid by cryoelectron tomography, Journal of virology, 2007, 81 (7), 3640-4.
Bortz Eric, Wang Lili, Jia Qingmei, Wu Ting-Ting, Whitelegge Julian P, Deng Hongyu, Zhou Z Hong, Sun Ren, Murine gammaherpesvirus 68 ORF52 encodes a tegument protein required for virion morphogenesis in the cytoplasm, Journal of virology, 2007, 81 (18), 10137-50.
Liu, F. and Zhou, Z.H., Comparative virion structures of human herpesviruses. In Human Herpesviruses: Biology, Therapy and Immunoprophylaxis, Arvin, A., Campadelli-Fiume, G., Moore, P., Mocarski, E., Roizman, B., Whitley, R., & Yamanishi, K., eds, Cambridge University Press, 2006, 27-43.
Zhou, Z. H.*, Stoops, JK, Krueger, Ultrastructure and Assembly of Human Herpesvirus-6 (HHV-6), In Human Herpesvirus-6, Krueger/Alashi (eds.), Elsevier Science Publishers, 2006, 11-21.
Yu, X Trang, P Shah, S Atanasov, I Kim, YH Bai, Y Zhou, ZH Liu, F, Dissecting human cytomegalovirus gene function and capsid maturation by ribozyme targeting and electron cryomicroscopy, Proceedings of the National Academy of Sciences of the United States of America, 2005, 102 (20), 7103-8.
Cong Yao, Jiang Wen, Birmanns Stefan, Zhou Z Hong, Chiu Wah, Wriggers Willy, Fast rotational matching of single-particle images, Journal of structural biology, 2005, 152 (2), 104-12.
Yu, X Shah, S Atanasov, I Lo, P Liu, F Britt, WJ Zhou, ZH, Three-dimensional localization of the smallest capsid protein in the human cytomegalovirus capsid, Journal of Virology, 2005, 79 (2), 1327-32.
Booth Christopher R, Jiang Wen, Baker Matthew L, Zhou Z Hong, Ludtke Steven J, Chiu Wah, A 9 angstroms single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope, Journal of structural biology, 2004, 147 (2), 116-27.
Wan, Y., Chiu, W., Zhou, Z.H., Full contrast transfer function correction in 3D cryo-EM reconstruction, IEEE Proceedings of ICCCAS, 2004, 960-964.
Qiao Ming, Ashok Mundrigi, Bernard Kristen A, Palacios Gustavo, Zhou Z Hong, Lipkin W Ian, Liang T Jake, Induction of sterilizing immunity against West Nile Virus (WNV), by immunization with WNV-like particles produced in insect cells, The Journal of infectious diseases, 2004, 190 (12), 2104-8.
Gu Yingqi, Zhou Z Hong, McCarthy Diane B, Reed Lester J, Stoops James K, 3D electron microscopy reveals the variable deposition and protein dynamics of the peripheral pyruvate dehydrogenase component about the core, Proceedings of the National Academy of Sciences of the United States of America, 2003, 100 (12), 7015-20.
Baker, ML Jiang, W Bowman, BR Zhou, ZH Quiocho, FA Rixon, FJ Chiu, W, Architecture of the herpes simplex virus major capsid protein derived from structural bioinformatics, Journal of molecular biology, 2003, 331 (2), 447-56.
Kong, Y., Ming, D., Wu, Y., Stoops, J.S., Zhou, Z.H., Ma, J., Conformational flexibility of pyruvate dehydrogenase complexes, J. Mol. Biol, 2003, 330 (1), 129-135.
Zhou, Z. H., Zhang, H., Jakana, J., Lu, X.Y., Zhang, J.Q., Cytoplasmic Polyhedrosis Virus Structure at 8 A by Electron ryomicoscopy: Structural Basis of Capsid Stability and mRNA Processing Regulation, Structure, 2003, 11 (6), 651-663.
Bortz, E. Whitelegge, J. P. Jia, Q. Zhou, Z. H. Stewart, J. P. Wu, T. T. Sun, R., Identification of proteins associated with murine gammaherpesvirus 68 virions, J Virol, 2003, 77 (24), 13425-32.
Lu, BY Atanasov, I Zhou, ZH Chang, JY, Reversible aggregation of mouse prion protein derivatives with PrPSC-like structural properties, Journal of protein chemistry, 2003, 22 (2), 115-26.
Zhou, Z.H.*, Stoops, J.K. and Reed, L.J., Structural and functional organization of pyruvate dehydrogenase complexes. In: Thiamine, Jordan F. & Patel M.S. (eds), Jordan F. & Patel M.S. (Eds), 2003, 309-330.
Xia, Q., Jakana, J., Zhang, J.-Q., Zhou, Z.H., Structural comparisons of empty and full CPV: protein-RNA interactions and implications for endogenous RNA transcription mechanisms, J. Biol. Chem, 2003, 278, 1094-1100.
Zhou, Z.H.* and Chiu, W., Structural determination of icosahedral viruses by electron cryomicroscopy at subnanometer resolution, Advances in Protein Chemistry, 2003, 64, 93-124.
Lo, P., Yu, X., Atanasov, I., Chandran, B., Zhou, Z.H., Three-dimensional localization of pORF65 in Kaposi’s sarcoma-associated herpesvirus capsid, J. Virol, 2003, 77, 4221-4230.
Yu, X.K., O’Connor, C.M., Atanasov, I., Damania, B., Kedes, D.H., Zhou, Z.H., Three-dimensional structures of the A, B, and C capsids of Rhesus monkey Rhadinovirus, J. Virol, 2003, 77, 13182-13193.
Dai, W Liang, Y Zhou, ZH, Web portal to an image database for high-resolution three-dimensional reconstruction, Journal of structural biology, 2003, 144 (1-2), 238-45.
Chiu, W., Baker, M.L., Jiang, W., Zhou, Z.H., Deriving folds of macromolecular complexes through electron cryomicroscopy and bioinformatics, Curr. Opin. Struct. Biol, 2002, 12 (2), 263-269.
Liang, Y., Ke, E.Y., Zhou, Z.H., IMIRS: a high-resolution 3D reconstruction package integrated with a relational image database, J. Struct. Biol, 2002, 137, 292-304.
Zhang, H Yu, XK Lu, XY Zhang, JQ Zhou, ZH, Molecular interactions and viral stability revealed by structural analyses of chemically treated cypovirus capsids, Virology, 2002, 298 (1), 45-52.