Protocols

Updated on Janunary 25, 2011

Protein Biochemistry

  • Affinity Purification of Protein Complexes
  • Preparation of GST Beads
  • Cytoplasmic Extracts fpr IP
  • GST Fusion Protein Prep
  • GST Fusion Protein Check
  • GST Pull Down
  • ImmunoPrecipitation
  • ImmunoBlotting

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    Transfection

  • Simplified calcium phosphate transfection / adherent cells
  • Electroporation and Luciferase assays for B cells
  • Luciferase assays in 293 cells
  • Transfection of BOSC 23 cells and infection of B-cells
  • Calcium phosphate transfection of BOSC cells for Viral Production

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    DNA-RNA Hybridization

  • cDNA Screen
  • Church Hybridization
  • FBI Hybridization
  • Oligo Hybridization
  • Zetabind Stripping

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    Plasmid Preps

  • Alkaline MiniPrep Bacterial Lysis
  • Plasmid MiniPrep/CTAB
  • Plasmid EasyPrep
  • Subcloning

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