Lysis Buffer: 10 mM Tris-HCl, PH 8.0, 1 mM EDTA, 15% wt/v Sucrose, 2
mg/ml
Lysozyme, 0.2 mg/ml Rnase A 0.1 mg/ml BSA (aliquids can be store at
-20 C freezer).
Proecdure:
Put 1.5 ml of overnight culture into a microcentrifuge tube
spin at 14 krpm for 2 min
suck off all the liquid
resuspend the pellet in 30 to 100 ul of lysis buffer
5 to 10 min at RT
boil for 1 min
spin at 14 krmp for 10 min
use 10 to 15 ul of supernant for each digestion.