Pick up bacterial colonies and and controls.
Grow in 2 ml of LBAmp for overnight at 37 C.
Take 25 ul of O/N culture and put into a 5 ml tube containing 0.5 ml LBAmp.
Incubate at 37 C for 2 hours.
Add 3 ul of 100 mM IPTG.
Incubate at 37 C for 2 hours.
Transfer 200 ul of the IPTG induced bacteria into a 1.5 ml tube.
Spin at 14 Krpm for 2 min.
Resuspend the pellet into 40 ul of 1 X SDS loading buffer containing 5% 2-ME.
Boil for 5 min.
Spin at 14 Krpm for 1 min.
Load on SDS-PAGE gel.
Stain with Coomassie blue.
Destain with 5% acetic acid and 20% methnol.
(You may speed up the staining and destaining procedures by microwave)