Solution:
1) STET: 8% sucrose, 50 mM Tris-HCl, Ph 8.0, 50 mM EDTA, 0.1% v/v Triton X-100
3) CTAB: 5% in 0.5M NaCl
4) RNase A: 10 mg/ml
5) 1.2 M NaCl
put 1.5 ml overnight culture into microcentrifuge tube
spin at 14 krpm for 2 min
suck off all the liquid
Take an aliquot of STET from the stock, add lysozyme to final concentration
of 2 mg/ml
add 200 ul of STET/Lysozyme to each tube
resuspend well
5 to 10 min at RT
boil the samples for 45 seconds
Spin at 14 krpm for 10 min
remove the debris by using tips and keep the supernatant
add 5 ul of Rnase and Mix
10 min at 68 C
add 10 ul CTAB and Mix
3 to 5 min at RT
spin at 14 krpm for 5 min
discard the supernatant
resuspend the pellet in 300 ul of 1.2 M NaCl
add 750 ul ETOH and Mix well
spin at 14 krpm for 10 min
rinse the pellet with 70 % ETOH
dry in the speed-vac for 2 min
resupend the plasmid DNA in 30 ul of H20
use 5 ul for cutting or sequencing.