Debi P. Nayak, B.V.Sc., Ph.D.
Professor
43-134 CHS
310-825-8558
310-825-8559

 

Research Interests

Major research effort in Dr. Nayak's lab is directed towards understanding the molecular basis of viral pathogenesis with special emphasis on pathogenesis, replication, assembly, morphogenesis and budding of influenza, Sendai and HIV viruses. Current projects involve defining (1) the function of different domains of influenza glycoproteins in polarized transport in epithelial cells; (2) the interaction among glycoproteins, the matrix protein and the viral RNP during assembly and budding; (3) functional basis of budding from the apical plasma membrane; (4) the steps involved in virus assembly at molecular level; (5) the role of matrix protein in budding and in viral pathogenesis; (6) the role of host proteins in virus budding.


Selected Publications

Sanderson, C. McQueen, N. and Nayak, D.P. (1993). Sendai virus assembly: M protein binds to viral glycoproteins in transit through the secretory pathway. J. Virol. 67:651-663.

Nayak, D.P., Rey, O. and Sanderson, C. (1993). Assembly and budding of orthomyxo-and paramyxovirus particles The role of matrix proteins. In:Option for the Control of Influenza II (Ed. A. Kendall and C. Hannoun) pp. 299-310. Elsevier, Amsterdam, The Netherlands.

Sanderson, C.M., Wu, H-H. and Nayak, D.P. (1994). Sendai virus M protein binds independently to either for HN glycoproteins in vivo. J. Virol. 68:69-76.

Kundu, A, and Nayak, D.P. (1994). Analysis of the signals for polarized transport of influenza virus (A/WSN/33) neuraminidase and human transferrin receptor, type II transmembrane proteins. J. Virol. 68:1812-1818.

Gujuluva, C.N., Kundu, A, Murti, K. G. and Nayak, D.P. (1994). Abortive replication of influenza virus A/WSN/33 in HeLa 229 cells: defective membrane function during entry and budding. Virology 204:491-505.

Biswas, S.K. and Nayak, D.P. (1994). Mutational analysis of the conserved motifs of influenza virus polymerase basic protein 1 (PB1), J. Virol. 68:1819-1826.

Hogue, B.G., and Nayak, D.P. (1994). Deletion mutation in the signal anchor domain activates cleavage of the influenza virus neuraminidase, a type II transmembrane protein. J. Gen.Virol. 75:1015-1022.

Nayak, D.P. Kundu, A. and Sanderson C.M. (1995). Analysis of membrane association in vivo and targeting of viral proteins in poloarized cells. Methods in Molecular Genetics 7:343-358 (Ed. K.W. Adolph). Acad. Press. NY.NY.

Sanderson, C.M., Avalos, R.T. Kundu, A. and Nayak, D.P. (1995). Interaction of Sendai viral F, HN and M Proteins with host cytoskeletal and lipid components in Sendai virus-infected BHK cells. Virology 209:701-707.

Kundu, A.K., Avalos, R.T. Sanderson, C.N. and Nayak, D.P.(1996). Transmembrane domain of influenza virus neuraminidase, a type II protein, possesses determinant for sorting to apical plasma membrane. J. Virol. 70: 6508-6515.

Nayak, D.P., (1996). Assembly and Morphogenesis of Orthomyxo-and Paramyxoviruses. ASM News 62:411-414.

Martin, R.A., and Nayak, D.P. (1996). Mutational analysis of HIV-1 gp160-mediated receptor interference: Intracellular Complex Formation. Virology. 220:461-472.

Martin, R.A., and Nayak, D.P. (1996). Membrane anchorage of gp160 is necessary and sufficient to prevent CD4 transport to the cell surface. Virology. 220:473-479.

Martin, R.A. and D.P. Nayak, (1996). Receptor interference mediated by the envelope glycoproteins of various HIV-1 and HIV-2 isolates. Virus Res. 45:135-145.

Biswas, S., and Nayak, D.P. (1996). Influenza virus basic protein polymerase 1 interacts with influenza virus basic protein polymerase 2 at multiple sites. J.Virol 70:6716-6722.

Avalos, R., Yu, Z. and Nayak, D.P. (1997). Association of influenza virus NP and M1 proteins with cellular cytoskeletal elements in influenza virus infected cells. J. Virol. 71:2947-2958.

Nayak, D.P. 1997. Influenza virus infection. In Encyclopedia of Human Biology (ed. R. Dulbecco). Vol. 4: 479-491.

Biswas, S.K., Boutz, P. L. and Nayak, D.P. (1998). Influenza virus nucleoprotein interacts with influenza virus polymerase proteins. J. Virol. 72:5493-5501.

Nayak, D.P. 2000. Viral morphology, replication and assembly. In viral Ecology (ed Chris Hurst), pp 63-124, Academic Press.

Ali, A., Avalos, R., Ponimaskin, E. and Nayak, D.P. (2000). Influenza virus assembly. The effect of influenza viral glycoproteins on the membrane-association of M1 protein. J. Virology 74:8709-8719.

Barman, S., and Nayak, D.P. (2000). Analysis of the transmembrane domain of influenza virus neuraminidase, a type II transmembrane glycoprotein, for apical sorting and raft association. J. Virology 74:6538-6545.

Ali, A and Nayak, D.P. (2000). Assembly of Sendai virus: M protein interacts with the cytoplasmic tail and transmembrane domain of F protein. Virology 276:289-303.

Barman, S., Ali, A., Hui, E. K.-W., Adhikari, L and Nayak, D.P. (2001). Transport of viral glycoproteins to the apical membranes and interaction of matrix protein with glycoproteins in the assembly of influenza virus. Virus Research 77:61-69.

Hui, E. K.-W., and Nayak, D.P. (2001). Role of ATP in influenza virus budding. Virology 290, 329-341.

Nayak, D.P. (2001). Virulence of 1918 pandemic influenza virus. ASM News 67:386-387.

Van Lenten, B.J., Wagner, A.C., Nayak, D.P., Hama, S. Navab, M., Fogelman, A.M. 2001. High-density lipoprotein loses its anti-inflammatory properties during acute influenza a infection. Circulation. 103: 283-2288.

Van Lenten BJ, Wagner AC, Anantharamaiah GM, Garber DW, Fishbein MC, Adhikary L, Nayak DP, Hama S, Navab M, Fogelman AM. Influenza infection promotes macrophage traffic into arteries of mice that is prevented byD-4F,an apolipoprotein A-I mimetic peptide. Circulation. 2002 Aug 27;106(9):1127-32.

Nayak, D.P. and Barman, S. (2002). Role of lipid raft assembly and budding site on viral pathogenesis. Advances in Virus Research. Invited review. Advances in Virus Research 8:1-28.

Nayak, D.P., and Hui, E.A. (2002). Assembly and Morphogenesis of Influenza virus. Recent Research Developments in Virology 4:35-54. Transworld Research Network.

Hui, E. K.-W., and Nayak, D.P. (2002). Role of G protein and protein kinse signaling in influenza virus budding in MDCK cells. J. Gen. Virol 83:3055-3066.

Barman,S.,Adhikary, L., Kawaoka, Y., and Nayak, D.P. 2003. Influenza A virus hemagglutinin containing basolateral localization signal does not alter the apical budding of a recombinant influenza virus in polarized MDCK cells. Virology 305:138-152.

Hui, Eric Ka-Wai, Subrata Barman, Tae Yong Yang and Debi P. Nayak. 2003. Basic residues of the helix six domain of influenza virus M1 involved in nuclear translocation of M1 can be replaced by PTAP and YPDL late domain motifs. J. Virol. 77:7078-7092.

Hui, Eric Ka-Wai ,Katherine Ralston, Amrit K. Judd and Debi P. Nayak. 2003. Conserved cysteine and histidine residues of the putative zinc finger motiff of influenza virus M1 protein are not critical for influenza virus replication. J. Gen. Virology 84:3105-3113.

Barman, S., Adhikary, L., Chakrabarti, A.K., Bernas, C., Kawaoka, Y., Nayak, D.P. (2004) Role of transmembrane domain and cytoplasmic tail amino acid sequences of influenza A virus neuraminidase in raft association and virus budding. J. Virol. 78:5258-5269.

Nayak DP, Hui EKW. (2004) The role of lipid microdomains in virus biology. In: Subcellular biochemistry, vol. 37, Membrane dynamics and domains (Quinn PJ, ed), chapter 14, pp.443-491. Kluwer Academic/Plenum Publishers, New York.

Hui ,E. K.-W., E. M. Yap, D. S. An, I. S. Y. Chen and D. P. Nayak (2004). Inhibition of influenza virus matrix (M1) protein expression and virus replication by U6 promoter-driven and lentivirus-mediated delivery of siRNA . J Gen Virol ; 85 (7): 1877-1884.

Nayak, D.P., Hui,E.K.-W. and Barman ,S.(2004) Assembly and budding of Influenza virus. Virus Research 106:147-165.

Van Lenten,B.J., Wagner,A.C., Navab,M., Anantharamaiah,G.M., Hui,E.K.-W., Nayak,D.P. Fogelman,A.M. (2004). D-4F,an apoA-I mimetic peptide, inhibits inflammatory response induced by influenza A virus infection of human type II pneumocytes Circulation 110:3252-3258.